Effect of ochratoxin A on the pathology and pathogenesis of hydropericardium syndrome in broiler chicken

D. Madhuri and P.C. Verma
Department of Veterinary Pathology
College of Veterinary Sciences, Haryana Agricultural University, Hisar - 125004

A study has been conducted to find out the effect of Ochratoxin A, on the pathology and pathogenesis of Hydropericardium syndrome (HPS) in broiler chicken. A total of 160, day old broiler chicks were divided into two groups i.e. XC and OC of 80 chicks each. The chicks in-group OC were given Ochratoxin A @ 1.5 ppm in the feed from day 1 till the end of the experiment whereas the birds in Group XC served as control. At the age of 21, chicks in both the groups were further divided into 2 subgroups XC & XH and OC & OH of 40 chicks each respectively. The birds in groups XH, and OH were inoculated with 0.5 ml of diluted HPSL inoculum subcutaneously at day 21 of age. Except for decreased growth rate in OC group no other clinical signs were observed in control groups. Following infection with HPSL inoculum chicks in infected groups exhibited clinical signs, which were more severe in group OH as compared to birds in XH group. A high mortality (60.60%) in-group OH where as moderate mortality (30.30 %) in-group XH was observed. Significant decrease in body weights in-group OC was observed as compared to control group. Within infected groups decrease in body weight was observed in both OH & XH groups though decrease was comparatively more in OH group birds. Serum enzymatic analysis showed significant decrease in TSP, albumin and globulins in OC group birds as compared to control group (XC). A significant decrease in TSP, albumin and increase in AST, ALT & LDH activities was observed in infected groups and it was comparatively more in OH group birds as compared to XH group birds. Immunological studies indicated decrease in humoral and cell-mediated immune response in infected groups as compared to their respective control groups. Grossly, the lesions were similar in both the infected groups though they persisted for long duration in OA treated birds. Significant increase in relative liver and spleen weight and decrease in bursa weight was observed in infected groups as compared to control groups. Histopatholgical alterations comprising basophilic I/N inclusion in the hepatocytes, depletion of lymphocytes in bursa, spleen, thymus, degenerative changes in kidney tubules and necrosis of myocardium were more severe and persisted for longer duration in OH group birds as compared to XH group birds. In conclusion, incorporation of Ochratoxin A in feed enhanced the severity and development of lesions in HPS infected birds.

Source : IPSACON-2005

Did You Like This Article? Bookmark it at Del.icio.us

Article Tools