Hatching percentage of donor eggs as per weight in ex-vivo embryo culture system

J.K. Chauhan, B.P. Singh, Meenal Rana, K.P. Singh and B.Singh
Central Avian Research Institute, Izatnagar- 243 122

Development of the chicken embryo culture technique from single cell stage to hatching opened the way to manipulate early stage embryos. The present study was conducted with an aim to improve the efficiency of ex-vivo embryo culture system to enhance the survivability and hatchability in the system using various techniques and modifications. A total of 233 fertile eggs of IC-3 strain were set in different experiment using ex-vivo embryo culture technique. Consequently, 134 eggs from same strain were also set in the incubator as control. Hatching percentage was calculated by dividing number of chicks hatched by total number of fertile eggs set in incubator multiplied by 100. The egg weight is positively correlated with early embryonic development, which may explain the differences in birds of different ages (Fasento, 1992). In the present study, to understand the effect of egg weight on hatchability of cultured chicks, the donor eggs were classified into 4 weight classes (46-50, 51-55, 56-60 and 61-65g). it was observed that the egg weight class having weight range from 51-55g and 56-60g, gave best hatching performance of 44.44% and 43.75% respectively in embryo culture system. While weight class 46-50 and 61-65 gm gave 33.33% and 32.25%. It can be concluded that the donor eggs having egg weight range from 51-60g, are likely to give highest hatchability performance in embryo culture system.

Source : IPSACON-2005

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