Transgenesis in considered as one of the important biotechnological tool, which allows introduction of new or altered genes into population of chicken of any other avian species. To develop this strategy a search has been made for ex-vivo embryo culture system for chicken that will optimise the uptake of foreign DNA into the embryonic cell. The investigation was undertaken with an aim to improve the efficiency of ex-vivo embryo culture system and to compare abnormalities in normal and embryo culture chicken. A total of 233 fertile eggs of IC-3, medium body size broiler populations aged between 32 to 35 weeks, obtained through natural mating were set in different experiments using ex-vivo embryo culture technique. Consequently, 134 eggs from same strain were also set in the incubator as control. A total of 97 and 112 chicks were hatched in former and later group respectively. During continuous observation the results revealed that percentage abnormal chicks produced in embryo culture system were 5.15% and 0.8% in control. The values of mortality just after hatch were 9.27% in EC and 3.57% in control. There was a peak of embryo loss in the few days prior to hatch. The overall post hatch survival rate was about 41.6% EC and 83.58% in control. The embryos that died late in development had a series of abnormalities (defects) that contributed to hatch. These included poor position in the shell, incomplete development of the chorio-allantoic membrane, residual albumen small size and incomplete retraction of the yolk sac. The results implied that turning of eggs during this period is critical for formation of sub-embryonic fluid.
Source : IPSACON-2005