Hatching observation in relation to orientation of embryo in ex-vivo embryo culture system

J.K. Chauhan, B.P. Singh, Meenal Rana, U.B. Singh and B. Singh
Central Avian Research Institute, Izatnagar- 243 122

The ex-vivo double window system was used for chicken embryo culture. The embryo in this system enables a more thorough analysis of embryonic development and physiology, since it allows continuous observation and access to the embryo, thus, it would be easier to study the development each and everyday. The present investigations was undertaken with an aim to improve the efficiency of the ex-vivo double window embryo culture system alongwith comparison of hatchability, growth rate, mortality and abnormalities in normal and embryo culture chicken. A total of 233 fertile eggs were set in different experiments using ex-vivo embryo culture technique, consequently, a control group was set. The present experiment was conducted to study the hatching observation in relation to orientation of embryo in ex-vivo embryo culture system between 20-21 days of incubation. The results revealed that almost 49.42% embryos were positioned on left (L), followed by dorsal surface upper most (Ds) as 23.71%, right side (R) orientation (21.65%), ventral surface (Vs) upper most (3.09%), and vertical (3.09%). The investigation concluded that only embryo culture system could provide the opportunity to see the development of same embryo continuously at each moment, during entire incubation period.

Source : IPSACON-2005

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