Molecular genetic characterization of White Leghorns using RAPD and microsatellite markers

Mahadeo Kumar, V.L.K. Prasad, R.P.Sharma, S.K.Mishra, B. Ramesh Gupta and G. Narasimha Rao Department of Animal Genetics and Breeding
College of Veterinary Science, Rajendranagar, Hyderabad 500 030, A.P.

A study was undertaken on IWI, IWH, IWF and IWD of White Leghorn and Dahlem Red strains undergoing selection for high egg production for 8 to 26 generations for molecular genetic characterization using RAPD (60 birds, 42 primers) and microsatellite markers (32 birds, 15 primers). Out of a total of 176 RAPD bands generated, 144 bands were polymorphic (81.82%). The primers PDP40F and PDP43F were useful to distinguish different layer strains. The mean intra-population genetic similarities based on band sharing and band frequencies ranged from 84.84 to 90.11 and 75.90 to 82.96 per cent, respectively, while the mean inter population genetic similarity varied from 56.82 to 88.54 per cent. The genetic distance between IWD and Dahlem Red was longest while the distance between IWF and IWD was the shortest. The mean number of alleles per locus, number of alleles among the strains and the effective number of alleles using the microsatellite markers varied from 3 to 6, 2.50 to 3.17 and 1.28 to 4.41, respectively. The mean PIC values for the loci ADL0040, ADL0152, ADL0371, ADL0376 and GCT0002 were 0.3613, 0.2683, 0.4935, 0.4150 and 0.4400, respectively. The estimates of unbiased and observed heterozygosity ranged from 0.313 to 0.426 and 0.152 to 0.253, respectively. The genetic distance between IWI and IWH was minimum (0.0195) and that between IWH and IWD was maximum (0.1958).

Source : IPSACON-2005

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