Multitrait selection with restriction for cutup carcass value: Genetic relatedness of strains involved based on randomly amplified polymorphic DNA

H. Khosravinia, H.N. Narasimha Murthy, K.P. Ramesh* and M. G. Govindaiah **
AICRP on poultry breeding for meat **Dept. of Animal Breeding Genetics & Biometrics
VeterinaryCollege, Hebbal, Bangalore 560024 * NDRI, Adugodi, Bangalore - 560030
E-mail: [email protected]

Five broiler strains, namely HC, HB × PB2, HB × PB2, PB2 and UM1, involving in a selection program and differing in selection intensity and genetic background, were screened for randomly amplified polymorphic DNA (RAPD) polymorphism using 10 selected decamer primers. Nine primers amplified the genomic DNA, generating 200 to 2500 bp and all detected polymorphism between strains. The mean number of within population scorable bands varied between 3.83 to 6.00, 3.92 to 6.50, 4.06 to 6.25, 4.09 to 5.60 and 3.60 to 5.40 in HC, HC ×PB2, HB × PB2, PB2 and UM1 genetic groups, respectively. Out of 74 bands scored using these primers, 34 (50.0%) were found to be polymorphic. The number of polymorphic loci ranged from 3-6 with an average of 4.33. Strains differed for within-population genetic similarity estimated by band frequency (WS= 93.55 to 99.25). Between-strain genetic similarity estimates, based on band sharing as well as on band frequency ranged from 71.35 to 86.45 and from 73.38 to 87.68, respectively. Genetic groups HC and PB2 were the most closely related to the other, while HB × PB2 and HC × PB2 appeared to be more distant from each other. The between-strain distance based on both band sharing and band frequency revealed the similar trends as for Between-strain genetic similarity. Based on BS and BF criteria, HB × PB2 and HC × PB2 as well as PB2 and UM1 strains can be merged to launch a new genetic group for further progress in biometrical objectives. A phylogenetic tree, derived using Nei’s coefficient of similarity revealed the different pattern of genetic distance between strains. In spite of effectiveness of RAPD markers to detect polymorphism between five genetic groups concerned, to bear out specificity and sequence homology of any possible strain specific bands could not be found using nine primers of concern and need further detailed study using more number of primers and hybridization, cloning and sequence experiments.

Source : IPSACON-2005

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