Measurement of genetic relatedness of naked neck and normally feathered birds through RAPD markers

B. Singh, B.P. Singh and U.B. Singh
National Central Avian Research Institute
Izatnagar - 243 122

Randomly amplified polymorphic DNA (RAPD) markers were employed to analyze the genetic polymorphism between naked neck and normally feathered birds in naked neck white (NNWP) and colored (NNCP) populations. The resulted polymorphism was further used in estimating the genetic similarity/genetic distance among six genotypes of two broiler populations. A total of seven random primers having high G-C Content (60-70%) were used to detect the polymorphism among the representative genotype pools. Out of 7, only 2 primers could detect the variability among the different genotypes. A total of 27 birds were amplified with these two primers and out of these, only two (one from each primer) was polymorphic. The genetic variability was of very low magnitude among the different genotypes of two populations. The present study revealed the use of RAPD markers in detecting the polymorphism and genetic relatedness of naked neck and normally feathered birds.

Source : IPSACON-2005

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