Generic differences between and within four avian species as assessed by Randomly Amplified Polymorphic DNA markers

Suryakant Mishra, Mahadeo Kumar*, R.P. Sharma and V. L. K Prasad*
Project Directorate on Poultry *Department of LPM, ANGRAU
Rajendranagar, Hyderabad - 500 030

Molecular genetic analysis were carried out on 4 important avian species viz. turkey, emu, duck and chickens (D. red) as sampled from the genetic stocks maintained a the department of Poultry science, ANGRAU and project directorate on poultry, Hyderabad, using Randomly amplified polymorphic DNA (RAPD) markers. Representative samples inclusive of both sexes from each of these species were subjected to RAPD analyses as per standard protocols, using 6 highly informative random primers (decamers) chosen from a total of 32 primers based on their polymorphic information content. A cumulative total of 161 polymorphic markers scored from these genetic samples were applied for making intra and inter generic comparison. The results revealed significantly variant and distinct fingerprint patterns specific to each of the species, which aided in instant differentiation of the species and the individuals within the respective species. Within-genera analysis of the samples indicated highest amount of genetic homogeneity for the D. Red (chicken) samples (84.7% genetic similarity), while the ducks were observed to be the least homogeneous as a genetic group (76.7%). Bulk of the RAPD alleles amplified from these primers were representative the variable regions of the respective genomes and less of the genomically homologous regions across the genera. As expected, the inter-generic distances were very high between the species. When measured on a relative scale, the chicken samples were at a maximum genetic distance from the Turkeys and the order of similarity for these species with chickens was: Turkey (0.193) ® Emus (0.242) ® Ducks (0.512). Obviously, the ducks were recorded to be the intermediates between the Turkeys and chicken vide their inter-genera genomic similarity in terms of their evolutionary time scale. The study proved that the RAPD markers were highly useful as genetic markers for inter-generic variability-assessments, which were efficiently reproducible, and an affordable marker sets for the purposes. It was also possible to develop genus-specific DNA markers from these RAPD loci for instant identification of the species, for various practical purposes.

Source : IPSACON-2005

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