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Effect of inclusion of herbal Ca and phytase on physical, chemical and radiological characteristics of tibia of commercial broiler chicken

Department of Animal Nutrition
W. B. University of Animal and Fishery Sciences
37 K.B. Sarani, Kolkata- 37



To study the effect of effect of inclusion of herbal Ca and phytase in the broiler feed by replacing di calcium phosphate (DCP) on physical, chemical and radiological characteristics of tibia, a 42-day trial using 280 Vencob broiler chicks was conducted. The chicks were randomly divided into seven treatment groups, each treatment consisting of two replicates. A corn Soya based experimental diets prepared for both starter and finisher to meet nutrient requirements as per NRC (1994) recommendation. The experimental groups were Control (C)-was offered basal diets, Treatment-1 (T 1) provided 50% DCP deficient diet compared to control, Treatmen-2 (T 2) was offered diet of T 1 with Herbal Ca (Mega Cal, marketed by Indian Herbs Pvt. Ltd.) @ 1 kg/ton, Treatment-3 (T 3)- offered diet of T 1 with phytase @ 0.5 kg/ton, Treatment-4 (T 4)- offered diet of T 1 with mega Cal (1 kg/ton) and phytase (0.5 kg/ton), Treatment-5 (T 5) provide mega Cal 0.5 kg/ton and Treatment-6 (T 6) provided mega Cal (0.5 kg/ton) and phytase (0.5 kg/ton).Effect of mega Cal on bone was assessed by physical (length and breadth), chemical (Ca, P, Cu, Zn, Mn and Fe) and radiological assay of tibia. Data analysis reveled that mineral concentration in Tibia, in terms of total ash percentage varied significantly (P<0.05) among the various treatments. Mineral concentration was highest in T 3 followed by T 2, T 6, C, T 1, T 4 and T 5. Ca concentration in bone was found highest in T 6 followed by T 2 and T 3. On statistical analysis no significant (P<0.01) difference was found in these three groups. Lowest Ca concentration was found in T 1 and varied significantly from all other treatments.

Data revealed that trend of variation between different groups on phosphorus was found accurately same as Ca concentration of bone. Zn concentration was found highest in T 4 followed by T 3 and both these groups have differed significantly (P<0.01) from other groups. T 2 differs significantly from T 6, T 5 and T 1. C and T 5 differed significantly from T 6 and T 1. Zn concentration was found lowest in T 1. Tibial length was highest (P<0.01) in T 5 followed by T 6, T 4, T 3 and C. Lowest tibial length was found in T 1.Tibial breadth revealed no significant (P<0.05) variation between T 5, T 4, T 6 and T3. Here T 5 showed highest value where T 1 was lowest. X- Ray photography of tibia of experimental birds of different treatment groups revealed poor growth and ossification in T 1, considering the depth of epiphysis, diaphysis and also the middle shaft of the bones in terms of ossification. It was clear that best bone growth was obtained in T 2 followed by T 5, T 6, T 4, C and T 3. Lowest bone growth was obtained in T 1. There was no indication of osteoporosis in the X- ray photography of bone. There was also no report of lameness during the experiment. So from the results it can be concluded that for substituting 50% of the total DCP, Herbal Calcium ( Mega Cal) @ 0.1 % in the feed can well be used for better bone development in broiler chicken production.

Source : IPSACON-2005
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