Effect of in ovo injection of glucose on the hatch weight and blood biochemical parameters of the day-old broiler chicks
S.K.BHANJA, A.B. MANDAL, S.K. AGARWAL AND S. MAJUMDAR
Central Avian Research Institute, Izatnagar - 243 122
In ovo injection of nutrients has been practiced for the manipulation of embryonic growth and gastrointestinal tract development in broiler and turkey. An experiment was conducted to study the effect of in ovo injection of glucose into the albumen and yolk sac of egg at different embryonic age on the hatch weight, hatchability and blood biochemical parameters. Fertile eggs (n= 325) from the broiler breeders maintained on the adequate nutritional plane were collected, weighed and distributed into 13 groups of 25 eggs each. One group was kept as control, which did not receive any injection. Six groups were injected with 50 mg of glucose either into albumen or yolk sac of the egg at 10, 14 and 18 th day of incubation. Rest six groups were injected with 0.5 ml of sterile water at the site and age specified above, which acted as sham control. In ovo injection of glucose on 10, 14 and 18 th d of incubation did not significantly influence the hatchability. However, injection on 14 and 18 th d had numerically higher hatchability than on 10 th d of incubation. Injection of glucose into the albumin of egg had higher (P<0.025) hatchability than into yolk sac. Though days of injection and deposition site did not affect the egg to chick weight ratio but glucose injected eggs had higher (P<0.009) chick weight than sham and un-injected control. In ovo injection of glucose on 10 and 14 th day of embryonic age had significantly higher plasma level of glucose (P<0.001) and protein (P<0.001) at the day-old than that injected on 18 th day of incubation. Glucose injection into yolk sac of embryo elevated plasma level of glucose (P<0.001) but lowered the protein level (P<0.001), whereas, injection into albumen of egg had reverse trend. Higher plasma level of uric acid (P<0.001) was recorded in the birds received glucose injection on 14 and 18 th day than 10 th day of incubation and un-injected control. At the same time glucose injection into yolk sac of embryo also elevated plasma level of uric acid (P<0.018). It may be concluded that in ovo injection of glucose significantly altered the chick growth and blood biochemical parameters.